The SonicMan is a high throughput multi-probe sonication instrument developed by MatriCal, Inc. configurable with 96, 384, and 1536 well formats. The Instrument is operated with a user-friendly touch screen interface. The SonicMan uses disposable gasketed pin lids to transfer sonic energy to each individual well and ensure no well to well cross contamination. Plates are placed on a extendable / retractable shuttle. The sonicater allows for power outputs between 100 and 1150 watts (12 watts/pin for 96 well formats and 3 watts/pin for 384 well formats at 100% power) and configurable time intervals from .1 to 20 seconds.
SonicMan DNA Shearing Application Note Download Now!
Features:
Stand-alone instrument
96, 384, and 1536 interchangeable disposable pinned lid technology
Plate shuttle allows for direct integration with pick & place robotics.
Variable power settings between 1 and 1150 watts.
Variable sonication time intervals from 0.1 to 20 seconds.
Applications:
Resuspend stored compounds in HTS
Resolubilize compounds in synthesis
Shear genomic DNA into small fragments for PCR amplification
Extract target proteins, RNA/DNA, enzymes via cell lysis
Mix low volume assays.
Drive sonochemical reactions
Transfect eukaryotic cells
Tissue homogenization
Software:
A user friendly touch screen provides a real-time graphical display depicting parameters such as cycles, on/off time, and power setting profiles.
Onboard password protected programs are easily defined using alphanumeric touch screen controls and simple create/save/edit functions
The SonicMan is equipped with a network card to connect via RS232 or Ethernet directly to PC, for integrated automated system.
Pin Lids :
The SonicMan uses disposable inert gasketed pin lids to transfer sonic energy to each individual wells and ensure no well to well cross contamination. Matrical pin lids are available in variable lengths to accommodate different well dimensions and your preferred microplate.
Dissolution of organic compounds in DMSO in HTS plate or tube format is a difficult problem as users move to higher compression plate formats. Precipitation of compounds from DMSO screening stocks is a recognized problem in the HTS materials management process. The adverse effect of freeze thaw cycles on DMSO stock solutions stored in plate format as a result of cherry picking operations has led to the gradual replacement of plate-based storage with tube-based storage so as to minimize the number of freeze thaw cycles. Compound solubility in DMSO is markedly decreased by uptake of small quantities of water. We attribute this effect to the non ideal properties of DMSO water mixtures such that cavity formation in solvent, a necessary step in dissolution, is more difficult in wet DMSO than in dry DMSO or in pure water. We report here that efficient compound dissolution is possible even in 384 well format by the use of in-well plate-based sonication. Surprisingly, compounds precipitated from DMSO stocks either by water uptake or repeated freeze thaw cycles can be re-dissolved by low energy sonication. Finally, we demonstrate that precipitation of compound from DMSO stock solutions is synergistically enhanced by water uptake into DMSO compound stock solutions as well as by increasing the number of freeze thaw cycles.
